Study on Preparation P(DMAA-co-AM) hydrogel and Immobilization α-Amylase

Zhi-jie GENG; Song-bai LIN; Gao-shuang HUANG; Ke-ping WANG

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Study on Preparation P(DMAA-co-AM) hydrogel and Immobilization α-Amylase

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- Study on Preparation P(DMAA-co-AM) hydrogel and Immobilization α-Amylase
- Polymer Bulletin Vol. 25, Issue 11, Pages: 69-75(2012)
- 作者机构：
  
  1.华侨大学材料科学与工程学院，厦门　361021
  2.黎明职业大学，泉州　362000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012-11，
  
  Received：21 May 2012，
  
  Revised：08 August 2012，
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Zhi-jie GENG, Song-bai LIN, Gao-shuang HUANG, Ke-ping WANG. Study on Preparation P(DMAA-co-AM) hydrogel and Immobilization α-Amylase. [J]. Polymer Bulletin 25(11):69-75(2012)
DOI：

Zhi-jie GENG, Song-bai LIN, Gao-shuang HUANG, Ke-ping WANG. Study on Preparation P(DMAA-co-AM) hydrogel and Immobilization α-Amylase. [J]. Polymer Bulletin 25(11):69-75(2012) DOI：

摘要

采用氧化还原引发体系

水溶液聚合法制备P(DMAA-co-AM)水凝胶

并以其作为载体固定α-淀粉酶。采用单一变量法研究交联剂(N

N’-亚甲基双丙烯酰胺)用量、引发剂(过硫酸铵)用量、单体浓度和单体配比对凝胶性能的影响。以水凝胶的最大平衡溶胀度为指标

得出制备P(DMAA-co-AM)水凝胶的优化条件:交联剂用量为0.3%

引发剂用量为0.6%

单体浓度为20%

N'-二甲基丙烯酰胺与丙烯酰胺的摩尔比为2:1。在优化条件下

采用原位聚合法固定α-淀粉酶

测定不同单体配比的P(DMAA-co-AM)水凝胶固定化α-淀粉酶的活性。

Abstract

Poly(N

N-dimethylacrylamide-co-acrylamide)hydrogel was prepared by aqueous solution polymerization in redox initiator system

and α-amylase was immobilized by this hydrogel. Effects of the amount of crosslinking agent and initiator

monomer concentration

as well as monomer ratio on the characteristic of hydrogel were studied. Maximum equilibrium swelling ratio as the index obtaine the optimizing condition of preparing Poly(N

N-dimethylacrylamide-co-acrylamide) hydrogel: the amount of crosslinking agent is 0.3%

the amount of initiator is 0.6%

monomer concentration is 20% and n(DMAA):n(AM) is 2:1.With this condition immobilize α-amylase in situ polymerization and study the activity of immobilized enzyme.

关键词

水凝胶平衡溶胀性α-淀粉酶固定化α-淀粉酶

Keywords

HydrogelEquilibrium swellingα-AmylaseImmobilized α-amylase

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