Study on Preparation and Specific Separation Properties of Boronate Affinity-based Surface Molecularly Imprinted Microspheres with Naringin as Template

LIN Ya-li; ZHANG Min-hui; QIU Xin-qi; LIU Yu; XU Wen-jie; WU Quan-zhou; HE Jian-feng

doi:10.14028/j.cnki.1003-3726.2024.24.085

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Study on Preparation and Specific Separation Properties of Boronate Affinity-based Surface Molecularly Imprinted Microspheres with Naringin as Template

更新时间：2024-09-24

- Study on Preparation and Specific Separation Properties of Boronate Affinity-based Surface Molecularly Imprinted Microspheres with Naringin as Template
- Polymer Bulletin Vol. 37, Issue 10, Pages: 1470-1481(2024)
- 作者机构：
  
  1.广州中医药大学中药学院，广州　 510006
  2.广东云浮中医药职业学院，云浮市中药(南药)资源开发利用与中药材GAP研究重点实验室，云浮　 527400
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2024.24.085
  CLC：
- Published：20 October 2024，
  
  Published Online：15 July 2024，
  
  Received：25 March 2024，
  
  Accepted：27 May 2024
- 稿件说明：
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林雅立, 张敏慧, 邱欣琪, 刘育, 徐文洁, 邬泉周, 何建峰. 表面硼酸亲和柚皮苷分子印迹微球的制备及其特异分离性能研究. 高分子通报, 2024, 37(10), 1470–1481

Lin, Y. L.; Zhang, M. H.; Qiu, X. Q.; Liu, Y. Xu, W. J.; Wu, Q. Z. He, J. F. Study on preparation and specific separation properties of boronate affinity-based surface molecularly imprinted microspheres with naringin as template. Polym. Bull. (in Chinese), 2024, 37(10), 1470–1481
林雅立, 张敏慧, 邱欣琪, 刘育, 徐文洁, 邬泉周, 何建峰. 表面硼酸亲和柚皮苷分子印迹微球的制备及其特异分离性能研究. 高分子通报, 2024, 37(10), 1470–1481 DOI： 10.14028/j.cnki.1003-3726.2024.24.085.

Lin, Y. L.; Zhang, M. H.; Qiu, X. Q.; Liu, Y. Xu, W. J.; Wu, Q. Z. He, J. F. Study on preparation and specific separation properties of boronate affinity-based surface molecularly imprinted microspheres with naringin as template. Polym. Bull. (in Chinese), 2024, 37(10), 1470–1481 DOI： 10.14028/j.cnki.1003-3726.2024.24.085.

摘要

以4-乙烯苯硼酸(VPBA)、甲基丙烯酸甲酯(MMA)为功能单体，乙二醇二甲基丙烯酸酯(EDMA)为交联剂，通过悬浮聚合法制得硼酸功能化聚合微球；并以此微球为载体，利用硼酸亲和作用，将柚皮苷模板分子锚定在微球表面，再通过盐酸多巴胺(DA)与聚乙烯亚胺(PEI)的氧化自聚共沉积作用在微球表面进行印迹涂覆，最后酸洗去除柚皮苷模板，制备得到表面硼酸亲和柚皮苷分子印迹微球(SMIMs)。利用扫描电子显微镜、激光共聚焦显微镜、水接触角实验、氮气吸附-脱吸附以及红外光谱对SMIMs进行表征，结果表明SMIMs表面形成了亲水性印迹薄膜。吸附实验结果表明，SMIMs在水相条件下具有较高的吸附容量、优异的特异选择性和较快的吸附动力学速率。固相萃取实验表明以SMIMs作为固相萃取柱吸附材料，能够有效地分离化橘红粗提物中的柚皮苷组分，其洗脱回收率为50.52%。

Abstract

Boric acid functionalized polymer microspheres were prepared

via

suspension polymerization by using methacrylate (MMA) and 4-vinylphenylboronic acid (VPBA) as functional monomers

and ethyleneglycol dimethacrylate (EDMA) as crosslinkers

respectively. Naringin template was covalently a

nchored onto the surface of the microspheres by boronate affinity

and then a thin coating layer of dopamine and polyethyleneimine was formed onto the microspheres surface

via

oxidative self-copolymerization. After removal of the template with an acidic solution

the naringin surface molecularly imprinted microspheres (SMIMs) based on boronate affinity were obtained. The resulting materials were characterized by scanning electron microscopy

laser confocal microscopy

water contact angle experiment

nitrogen adsorption/desorption and infrared spectroscopy. The results show that the hydrophilic imprinted film has been successfully formed on the surface of boric acid functionalized polymer microspheres (BPMs). The adsorption experiments indicate that the SMIMs possess high adsorption capacity

excellent specific selectivity and fast adsorption kinetic rate toward template under water phase conditions. In addition

the extraction experiments reveal that the SMIMs as the adsorbed materials of solid phase extraction (SPE) can effectively separate naringin from

Exocarpium Citri Grandis

extract

and the total recovery of elution solution can reach 50.52%.

关键词

柚皮苷硼酸亲和表面印迹微球多巴胺共涂覆萃取分离

Keywords

NaringinBoronate affinitySurface imprinted microspheresDopamine co-coatingExtraction separation

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