Rolling Circle Amplification Based Smart DNA Hydrogels: From Fabrication to Biomedical Applications

ZHANG Rui; SHEN Xing-hong; YAO Chi; YANG Da-yong

doi:10.14028/j.cnki.1003-3726.2025.25.273

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Rolling Circle Amplification Based Smart DNA Hydrogels: From Fabrication to Biomedical Applications

更新时间：2026-01-16

- Rolling Circle Amplification Based Smart DNA Hydrogels: From Fabrication to Biomedical Applications
- Polymer Bulletin Vol. 39, Issue 1, Pages: 20-38(2026)
- 作者机构：
  
  复旦大学化学系　聚合物分子工程全国重点实验室　上海 200438
  天津大学　合成生物技术全国重点实验室　合成生物学前沿科学中心　系统生物工程教育部重点实验室　合成生物与生物制造学院　天津 300350
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2025.25.273
  CLC：
- Received：12 September 2025，
  
  Accepted：19 October 2025，
  
  Published Online：10 December 2025，
  
  Published：20 January 2026
- 稿件说明：
移动端阅览
张睿, 沈星宏, 姚池, 仰大勇. 基于滚环扩增反应的智能DNA水凝胶构建及其生物医用进展. 高分子通报, 2026, 39(1), 20–38.

Zhang, R.; Shen, X. H.; Yao, C.; Yang, D. Y. Rolling circle amplification based smart DNA hydrogels: from fabrication to biomedical applications. Polym. Bull. (in Chinese), 2026, 39(1), 20–38.
张睿, 沈星宏, 姚池, 仰大勇. 基于滚环扩增反应的智能DNA水凝胶构建及其生物医用进展. 高分子通报, 2026, 39(1), 20–38. DOI： 10.14028/j.cnki.1003-3726.2025.25.273.

Zhang, R.; Shen, X. H.; Yao, C.; Yang, D. Y. Rolling circle amplification based smart DNA hydrogels: from fabrication to biomedical applications. Polym. Bull. (in Chinese), 2026, 39(1), 20–38. DOI： 10.14028/j.cnki.1003-3726.2025.25.273.

摘要

DNA水凝胶具有序列可编程性、分子识别能力、刺激响应性、生物相容性和生物降解性等独特性质，广泛应用于材料科学与生物医用领域。滚环扩增(rolling circle amplification，RCA)反应是一种简单高效的等温酶促扩增策略，可合成具有大量重复功能单元的超长DNA单链。基于RCA技术，研究人员构建了一系列智能DNA水凝胶材料，具有广阔的生物医学应用前景。本文综述了通过RCA反应制备具有多功能的智能DNA水凝胶的方法，含多聚适配体的DNA水凝胶和生物颗粒的特异性识别与分离，及其后续在疾病诊疗领域的应用研究。

Abstract

DNA hydrogels exhibit unique properties such as sequence programmability

molecular recognition capability

stimuli-responsiveness

biocompatibility

and biodegradability

which have enabled their extensive applications in materials science and biomedical fields. Rolling circle amplification (RCA)

a simple and efficient isothermal enzymatic amplification strategy

can synthesize ultra-long single-stranded DNA with numerous repeated functional units. Leveraging RCA technology

researchers have developed a series of smart DNA hydrogel materials with promising potential for biomedical applications. This review summarizes the methodologies for fabricating multifunctional DNA hydrogels

via

RCA reaction

discusses the interactions between polyvalent aptamer-incorporated DNA hydrogels and biological particles upon specific recognition

and highlights their applications in the field of disease therapy and diagnosis.

关键词

Keywords

references

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