Structural Characterization and Its Free Radical Effect of a Water-soluble Polysaccharide from Strigaasiatica (L. )O. Ktze.

Kun WANG; Na A-XI; Mo LI; Chun-lan LIU

doi:10.14028/j.cnki.1003-3726.2018.11.006

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Structural Characterization and Its Free Radical Effect of a Water-soluble Polysaccharide from Strigaasiatica (L. )O. Ktze.

更新时间：2022-09-05

- Structural Characterization and Its Free Radical Effect of a Water-soluble Polysaccharide from Strigaasiatica (L. )O. Ktze.
- Polymer Bulletin Vol. 31, Issue 11, Pages: 52-59(2018)
- 作者机构：
  
  1.中央民族大学生命与环境科学学院，北京　100081
  2.北京市食品环境与健康工程技术研究中心，北京　100081
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2018.11.006
  CLC：
- Published：20 November 2018，
  
  Received：01 May 2018，
  
  Revised：10 September 2018，
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Kun WANG, Na A-XI, Mo LI, Chun-lan LIU. Structural Characterization and Its Free Radical Effect of a Water-soluble Polysaccharide from Strigaasiatica (L. )O. Ktze.. [J]. Polymer Bulletin 31(11):52-59(2018)
DOI：

Kun WANG, Na A-XI, Mo LI, Chun-lan LIU. Structural Characterization and Its Free Radical Effect of a Water-soluble Polysaccharide from Strigaasiatica (L. )O. Ktze.. [J]. Polymer Bulletin 31(11):52-59(2018) DOI： 10.14028/j.cnki.1003-3726.2018.11.006.

摘要

独脚金粗多糖（Strigaasiatica （L. ）O. Ktze. polysaccharide， SKPc）经 DEAE-Sephadex A-25 以及Sephadex G-100柱层析分离纯化后得到独脚金多糖组分（SKP

* ）。采用高效液相色谱（HPLC）法检测SKP

*，表明多糖SKP

*极性、相对分子质量是均一的，且其相对分子质量为14852u。采用气相色谱（GC）分析法研究SKP

*的单糖组成为葡萄糖（Glu）、阿拉伯糖（Ara）、半乳糖（Gal）、木糖（Xyl）、甘露糖（Man）、鼠李糖（Rha），相对摩尔比是2. 5：2.0：0.85：0.4：0. 5：1.0；可能主要以β型糖苷键连接。采用化学分析和仪器分析结合进行SKP

*结构分析，表明SKP

*结构是Ara以β-（1→3）糖苷键连接构成主链之一，2，4或2，5位有支链；Gal以β-（1—6）糖苷键连接构成SKP

*的主链之一，且在3位上有分枝。支链由β-（1→4或1→5）Xyl、β-（1→4，1→6，1 →2）Glc构成。Rha、Ara、Man、Gal、Glc都有一部分构成糖分子的末端残基。体外清除羟基实验结果表明，SKP

*有较好地清除羟基自由基的活性。

Abstract

SKPc was extracted from Strigaasiatica ( L. ) O. Ktze. SKPc which was deproteinized and decolorized was isolated and purified using DEAE-Sephadex A-25 and Sephadex G-100 and got SKP: . High performance liquid chromatography ( HPLC) indicated that SKP

* was a homogeneity polysaccharide

and the average molecular weight of SKP

* was approximately 14852 u. The water-soluble polysaccharide SKP

* has been analyzed by GC

it was composed of Glu

Ara

Gal

Xyl

Man

Rha

with a molar ratio of 2. 5:2. 0:0. 85:0. 4:0. 5:1. 0. The most of the sugar residues were existed as β-configuration. And the main chain was made up of β-(1→3)Ara

and β-(1→6)Gal

on average. The side chain was composed of β- (1→4 or 1→5) -Rha and (1→6

1→4

1→2) -Glc. The terminal residue was composed of Ara

Man

Gal and Glc. The scavenging rates of the SKP

* on hydroxyl was good.

关键词

独脚金多糖分离纯化结构表征自由基

Keywords

Strigaasiatica(L. )O. KtzePolysaccharideIsolation purificationStructural characterizationFree radical

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