N-dimethylacrylamide-co-acrylamide)hydrogel was prepared by aqueous solution polymerization in redox initiator system
and α-amylase was immobilized by this hydrogel. Effects of the amount of crosslinking agent and initiator
monomer concentration
as well as monomer ratio on the characteristic of hydrogel were studied. Maximum equilibrium swelling ratio as the index obtaine the optimizing condition of preparing Poly(N
N-dimethylacrylamide-co-acrylamide) hydrogel: the amount of crosslinking agent is 0.3%
the amount of initiator is 0.6%
monomer concentration is 20% and n(DMAA):n(AM) is 2:1.With this condition immobilize α-amylase in situ polymerization and study the activity of immobilized enzyme.