Small Angle X-ray scattering (SAXS) is a universal technique to study the submicroscopic structures of polymers including proteins. As one of the most available biomaterials

regenerated silk fibroin (RSF) with outstanding biocompatibilities has great potential applications in biomedical materials as well as biomimetic materials. RSF materials

such as film and fiber

are normally processed from its solution. To improve the properties of RSF materials

it is necessary to understand the microscopic/submicroscopic structures of RSF before and after processing. This paper introduced SAXS methods for concentrated RSF solution to obtain the aggregation structure parameters and construct the 3D model of RSF solution. The structure parameters of RSF film were obtained from correlation function by using Debye-Bueche method. The long period

interface and orientation of RSF fibers were calculated by applying Ruland method.