脱细胞肝基质复合丝素蛋白水凝胶支架的制备及表征

雷敏; 张苗; 尹诗韵; 孙中涛; 刘心雨; 付开秀; 牛晓; 陈国宝

doi:10.14028/j.cnki.1003-3726.2024.24.179

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脱细胞肝基质复合丝素蛋白水凝胶支架的制备及表征

研究论文 | 更新时间：2024-12-12

- 脱细胞肝基质复合丝素蛋白水凝胶支架的制备及表征
- Preparation and Characterization of Decellularized Liver Matrix Composite Silk Fibroin Hydrogel Scaffold
- 高分子通报 2024年37卷第12期页码：1791-1800
- 作者机构：
  
  重庆理工大学药学与生物工程学院，重庆 400054
- 作者简介：
  
  *陈国宝，E-mail: gbchen@cqut.edu.cn
- 基金信息：
  
  重庆市教委科学技术研究项目(KJQN202301136);大学生创新创业训练计划项目(重庆市级)
- DOI：10.14028/j.cnki.1003-3726.2024.24.179
  中图分类号：
- 纸质出版日期：2024-12-20，
  
  网络出版日期：2024-09-12，
  
  收稿日期：2024-06-15，
  
  录用日期：2024-08-17
- 稿件说明：
移动端阅览
雷敏, 张苗, 尹诗韵, 孙中涛, 刘心雨, 付开秀, 牛晓, 陈国宝. 脱细胞肝基质复合丝素蛋白水凝胶支架的制备及表征. 高分子通报, 2024, 37(12), 1791–1800

Lei, M.; Zhang, M.; Yin, S. Y.; Sun, Z. T.; Liu X. Y.; Fu, K. X.; Niu, X.; Chen, G. B. Preparation and characterization of decellularized liver matrix composite silk fibroin hydrogel scaffold. Polym. Bull. (in Chinese), 2024, 37(12), 1791–1800
雷敏, 张苗, 尹诗韵, 孙中涛, 刘心雨, 付开秀, 牛晓, 陈国宝. 脱细胞肝基质复合丝素蛋白水凝胶支架的制备及表征. 高分子通报, 2024, 37(12), 1791–1800 DOI： 10.14028/j.cnki.1003-3726.2024.24.179.

Lei, M.; Zhang, M.; Yin, S. Y.; Sun, Z. T.; Liu X. Y.; Fu, K. X.; Niu, X.; Chen, G. B. Preparation and characterization of decellularized liver matrix composite silk fibroin hydrogel scaffold. Polym. Bull. (in Chinese), 2024, 37(12), 1791–1800 DOI： 10.14028/j.cnki.1003-3726.2024.24.179.

摘要

近年来，脱细胞基质在组织工程中展现出巨大应用潜力，但如何改善其力学性能仍然是一个问题。为了探究引入丝素蛋白(silk fibroin

SF)改善脱细胞肝基质(decellularized liver matrix

DLM)力学性能的复合水凝胶支架用于肝组织工程的潜能，对大鼠肝脏进行脱细胞处理后，与SF溶液按等浓度不同体积比例混合交联，制成4组SF/DLM复合支架，SF与DLM溶液的比例分别为100:0、75:25、50:50、25:75，并对支架的形态结构、体外降解性、亲水性、力学性能和细胞相容性等进行了表征。研究结果表明SF能够改善复合支架的力学性能，SF比例的增加使支架的孔隙率和抗压强度提高，支架的降解性能和亲水性能得到提升，结合DLM的促细胞生成功能，该复合支架有望应用于肝修复组织工程。

Abstract

In recent years

decellularized matrix has shown great potential in tissue engineering

but how to improve its mechanical properties is still a problem. In order to explore the potential of using a composite hydrogel scaffold with silk fibroin (SF) to improve the mechanical properties of decellularized liver matrix (DLM) for liver tissue engineering

four groups of SF/DLM composite scaffolds were prepared by cross-linking the rat liver with SF solution at the same concentration and different volume proportions. The proportions of SF to DLM solution were 100:0

75:25

50:50

25:75

respectively. The morphological structure

in vitro

degradability

hydrophilicity

mechanical properties and cell compatibility of the scaffolds were characterized. The results showed that SF could improve the mechanical properties of the composite scaffold

the porosity

pressure resistance

degradability and hydrophilicity of the scaffold were all improved. Combined with the cell promoting function of DLM

the composite scaffold is expected to be applied in liver r

epair tissue engineering.

关键词

支架材料脱细胞基质丝素蛋白肝组织工程

Keywords

Scaffold materialDecellularized matrixSilk fibroinLiver tissue engineering

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