微环境响应铜氮掺杂纳米平台用于化学动力学抗菌的研究

张丽珍; 张利娜; 杨冬业; 黄夕洋; 杨婷

doi:10.14028/j.cnki.1003-3726.2025.25.179

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微环境响应铜氮掺杂纳米平台用于化学动力学抗菌的研究

研究论文 | 更新时间：2025-10-30

- 微环境响应铜氮掺杂纳米平台用于化学动力学抗菌的研究
- Microenvironment-responsive Copper, Nitrogen-Doped Nanoparticle Platform for Chemodynamic Antibacterial Research
- 高分子通报 2025年38卷第11期页码：1659-1670
- 作者机构：
  
  1.桂林师范学院化学与药学系，桂林 541001
  2.桂林医科大学药学院，桂林 541004
  3.广西壮族自治区中国科学院广西植物研究所，桂林 541006
- 作者简介：
  
  E-mail: 112005046@glmc.edu.cn
  E-mail: hxy@gxib.cn
- 基金信息：
  
  国家重点研发计划(2022YFD1600302);广西科技重大专项(桂科AA22096020);桂林市科学研究与技术开发计划(20230132-1);桂林市科研技术发展计划(20232C20681923);桂林师范高等专科学校高层次人才项目(GCC202207)
- DOI：10.14028/j.cnki.1003-3726.2025.25.179
  中图分类号：
- 收稿：2025-06-18，
  
  录用：2025-08-21，
  
  网络出版：2025-09-25，
  
  纸质出版：2025-11-20
- 稿件说明：
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张丽珍, 张利娜, 杨冬业, 黄夕洋, 杨婷. 微环境响应铜氮掺杂纳米平台用于化学动力学抗菌的研究. 高分子通报, 2025, 38(11), 1659-1670.

Zhang, L. Z.; Zhang, L. N.; Yang, D. Y.; Huang, X. Y.; Yang, T. Microenvironment-responsive copper, nitrogen-doped nanoparticle platform for chemodynamic antibacterial research. Polym. Bull. (in Chinese), 2025, 38(11), 1659-1670.
张丽珍, 张利娜, 杨冬业, 黄夕洋, 杨婷. 微环境响应铜氮掺杂纳米平台用于化学动力学抗菌的研究. 高分子通报, 2025, 38(11), 1659-1670. DOI： 10.14028/j.cnki.1003-3726.2025.25.179.

Zhang, L. Z.; Zhang, L. N.; Yang, D. Y.; Huang, X. Y.; Yang, T. Microenvironment-responsive copper, nitrogen-doped nanoparticle platform for chemodynamic antibacterial research. Polym. Bull. (in Chinese), 2025, 38(11), 1659-1670. DOI： 10.14028/j.cnki.1003-3726.2025.25.179.

摘要

抗生素耐药性对人类健康构成重大威胁，因此，开发有效的替代抗菌剂十分必要。化学动力学疗法(chemodynamic therapy，CDT)因细菌耐药性极低、抗菌效率较高而被广泛研究。本研究开发了一种响应细菌微环境的铜氮掺杂纳米平台，该平台利用细菌微环境中过表达产生的过氧化氢，通过类芬顿反应产生高毒性羟基自由基，从而抑制细菌生长。通过抑菌生长曲线实验、最低抑菌浓度(minimum inhibitory concentration，MIC)和最小杀菌浓度(minimum bactericidal concentration，MBC)测定，以及打孔法和平板法抑菌实验，表明制备的铜氮掺杂碳点对革兰氏阳性金黄色葡萄球菌(

Staphylococcus aureus，S. aureus

)和革兰氏阴性大肠杆菌(

Escherichia coli

，

E. coli

)均具有良好的杀菌效果。本研究为设计与开发新型抗菌剂提供了重要参考。

Abstract

Antibiotic resistance poses a significant threat to human health

necessitating the development of effective alternative antibacterial agents. Chemodynamic therapy (CDT) has been extensively studied because of its minimal bacterial resistance and high antibacterial efficiency. In this study

a copper-nitrogen co-doped carbon dot platform was developed to respond to bacterial microenvironments. By utilizing overexpressed hydrogen peroxide in the bacterial microenvironment

the platform generates highly toxic hydroxyl radicals through Fenton-like reactions

thereby inhibiting bacterial growth.

In vitro

assays

including bacterial growth inhibition curves

minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination

as well as punch method and agar plate tests

demonstrated the potent antibacterial activity of the copper-nitrogen co-doped carbon dot against both gr

am-positive (

Staphylococcus aureus

S. aureus

) and gram-negative (

Escherichia coli

E. coli

) strains. This study provides a rational design strategy for microenvironment-responsive antibacterial agents based on metal-doped nanomaterials.

关键词

Keywords

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