Construction and in vitro Evaluation of 5-Fluorouracil-and Gene-loaded PLGA/O-CMC Nanoparticles

Yun-xia HU; Xu-bo YUAN; Chun-sheng KANG; Yi GUO; Jin CHANG

doi:10.14028/j.cnki.1003-3726.2004.04.008

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Construction and in vitro Evaluation of 5-Fluorouracil-and Gene-loaded PLGA/O-CMC Nanoparticles

更新时间：2022-09-18

- Construction and in vitro Evaluation of 5-Fluorouracil-and Gene-loaded PLGA/O-CMC Nanoparticles
- Polymer Bulletin Vol. 17, Issue 4, Pages: 69-73(2004)
- 作者机构：
  
  天津大学材料科学与工程学院纳米生物技术研究所，天津　300072
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2004.04.008
  CLC：
- Published：2004-08，
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Yun-xia HU, Xu-bo YUAN, Chun-sheng KANG, Yi GUO, Jin CHANG. Construction and in vitro Evaluation of 5-Fluorouracil-and Gene-loaded PLGA/O-CMC Nanoparticles. [J]. Polymer Bulletin 17(4):69-73(2004)
DOI：

Yun-xia HU, Xu-bo YUAN, Chun-sheng KANG, Yi GUO, Jin CHANG. Construction and in vitro Evaluation of 5-Fluorouracil-and Gene-loaded PLGA/O-CMC Nanoparticles. [J]. Polymer Bulletin 17(4):69-73(2004) DOI： 10.14028/j.cnki.1003-3726.2004.04.008.

摘要

以聚乳酸-乙醇酸共聚物(PLGA)和自行制备的

-羧甲基壳聚糖(

-CMC)为原料，以5-氟尿嘧啶(5-Fu)为抗癌药物模型，以反义EGFR(表皮生长因子受体)为基因药物模型，构建与评价了同载抗癌药物与基因的复合功能纳米药物载体系统。同载超微粒子的平均粒径为258.7nm，粒径分布指数为0.142，粒子表面ξ电位为-10.67eV。同载超微粒子在PBS 中的释药行为研究表明：超微粒子中5-FU 和基因均具有零级缓慢释放特性。体外肿瘤细胞存活率实验和免疫组化实验均证实同载超微粒子能高效抑制TJ905人脑胶质瘤细胞的增殖。最后用荧光共聚焦显微镜动态监测了超微粒子进入瘤细胞的转染过程，发现粒子可在不同时间内进入细胞浆和细胞核。

Abstract

5-Fluorouracil (5-FU) and antisense-EGFR cDNA loaded nanoparticles were prepared by improved W/O/W method using poly (D

L-lactide-co-glycolic acid) (PLGA) and

-carboxymethyl chitosan (O-CMC) due to their attractive degradation and physicochemical properties

and this multiple-functional drug delivery system can realize the combination effect of anticancer drug and gene in the therapy of diseases.The results of characterizing these nanoparticles showed that:the mean size

polydispersity and Zeta potenial of 5- FU-encapsulated PLGA/

-CMC NPs while absorbing antisense-EGFR was 258.7nm

0.142

and -10.67eV

respectively.The release behavior of 5-FU and gene from NPs was coincidence with Zero-level release. Gliomas cell viability in vitro as well as immunohistochemical staining demonstrated that these nanoparticles had high cytotoxicity on gliomas cells TJ905.Fluorescence confocal microscope was used to trace the procedures of cell transfection induced by nanoparticles.It was found that nanoparticles were both in cytoplasma and nucleus after 24h.

关键词

O-羧甲基壳聚糖聚乳酸-乙醇酸共聚物超微粒子抗癌药物基因治疗缓释

Keywords

O-CMCPLGANanoparticlesAnti-cancer drugsGene TherapySustained Release

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