<sup>99</sup>mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution

Jie LIU; Chun-sheng KANG; Jian TAN; Xu-bo YUAN; Fu-hai ZHANG; En-jiang TIAN; Pei-yu PU; Jing SHENG

doi:10.14028/j.cnki.1003-3726.2006.01.006

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⁹⁹mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution

更新时间：2022-09-18

- ⁹⁹mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution
- Polymer Bulletin Vol. 19, Issue 1, Pages: 39-43(2006)
- 作者机构：
  
  1.天津大学材料科学与工程学院，天津　30072
  2.天津医科大学总医院神经外科研究室，天津　300052
  3.天津医科大学核医学室，天津　300052
  4.天津医科大学免疫学研究室，天津　300052
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2006.01.006
  CLC：
- Published：2006-01，
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Jie LIU, Chun-sheng KANG, Jian TAN, Xu-bo YUAN, Fu-hai ZHANG, En-jiang TIAN, Pei-yu PU, Jing SHENG. ⁹⁹mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution. [J]. Polymer Bulletin 19(1):39-43(2006)
DOI：

Jie LIU, Chun-sheng KANG, Jian TAN, Xu-bo YUAN, Fu-hai ZHANG, En-jiang TIAN, Pei-yu PU, Jing SHENG. ⁹⁹mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution. [J]. Polymer Bulletin 19(1):39-43(2006) DOI： 10.14028/j.cnki.1003-3726.2006.01.006.

摘要

以转铁蛋白溶液为外水相，聚乳酸丙酮溶液为油相，纳米沉淀法制备了表面结合转铁蛋白的聚乳酸纳米微粒，以二氯亚锡为还原剂，直接法和CDPTA 螯合法对纳米微粒进行

mTc 放射性标记，以C6胶质瘤细胞实验考察了标记对纳米微粒表面转铁蛋白活性的影响，结果表明直接法标记率较高，大于80.1%，对转铁蛋白活性有影响。CDPTA 螯合法标记法较低（72.3%），对转铁蛋白活性影响较小。以脑部荷胶质瘤大鼠为动物模型，鼠尾静脉注射放射性标记纳米微粒，SPECT 示踪和γ计数器检测显示：以转铁蛋白表面修饰的聚乳酸纳米微粒经静脉注射后主要分布于肝、脾，与正常鼠相比，荷胶质瘤大鼠对纳米微粒的摄取率有所提高。

Abstract

PLA nanoparticles coated with transferrin were prepared by nano-precipitation using transferrin liquor as external aqueous phase.The nanoparticles were radiolabeled by two methods

i.e.

directly labeling by

mTc and DTPAA chelate indirectly labeling

using SnCl

as reducing agent.The activity of transferrin on the surface of PLA nanoparticles radiolabeled was studied by C6 glioma cell experiment in vitro

and the results showed that The activity of transferrin was damaged when directly deoxidized with SnCl

whereas DTPAA indirectly labeling has little effect on the transferrin activity.The biodistribution of radiolabeled nanoparticles was investigated by SPECT and γcounter technologies.The distributions of surface-containing transferrin PLA nanoparticles were concentrated mainly at liver and spleen after intravenous injection.When seeding the brain with C6glioma cell

the brain ingestion of surfacecontaining transferrin PLA nanoparticles was slightly increased.

关键词

纳米微粒转铁蛋白表面放射性标记体内分布

Keywords

NanoparticlesTransferrinSurfaceRadiolabelingBiodistribution

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Institute of Polymer Science, Zhejiang University

Department of Polymer Science, Zhejiang University

Institute of Nanobiotechnology,School of material science and engineering,Tianjin University

Department of Chemistry,Shanghai University

Department of Inorganic Materials,Shanghai University

⁰

99mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution

DOI：10.14028/j.cnki.1003-3726.2006.01.006

摘要

Abstract

关键词

Keywords

references

⁹⁹mTc Radiolabeling of PLA nanoparticles Surface-modified by Transferrin and their Biodistribution