Influence of the Concentrations of Hyaluronic Acid on the Adhesion and Proliferation of Human Fibroblasts

Hai-feng LIU; Jin CHANG; Yi-ping CHEN; Kang-de YAO

doi:10.14028/j.cnki.1003-3726.2006.06.009

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Influence of the Concentrations of Hyaluronic Acid on the Adhesion and Proliferation of Human Fibroblasts

更新时间：2022-09-18

- Influence of the Concentrations of Hyaluronic Acid on the Adhesion and Proliferation of Human Fibroblasts
- Polymer Bulletin Vol. 19, Issue 6, Pages: 68-74(2006)
- 作者机构：
  
  天津大学材料科学与工程学院，天津　300072
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2006.06.009
  CLC：
- Published：2006-06，
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Hai-feng LIU, Jin CHANG, Yi-ping CHEN, Kang-de YAO. Influence of the Concentrations of Hyaluronic Acid on the Adhesion and Proliferation of Human Fibroblasts. [J]. Polymer Bulletin 19(6):68-74(2006)
DOI：

Hai-feng LIU, Jin CHANG, Yi-ping CHEN, Kang-de YAO. Influence of the Concentrations of Hyaluronic Acid on the Adhesion and Proliferation of Human Fibroblasts. [J]. Polymer Bulletin 19(6):68-74(2006) DOI： 10.14028/j.cnki.1003-3726.2006.06.009.

摘要

透明质酸广泛存在于细胞外基质、细胞表面和细胞内部，其独特的理化特性使其具有多种生物学功能。透明质酸对细胞具有多种作用，可以调控细胞生长因子和细胞因子的分泌，抑制蛋白酶的分解，影响细胞的黏附、生长、增殖和分化。本实验将不同浓度的透明质酸加入成纤维细胞培养液中，考察了在培养液中透明质酸的浓度对成纤维细胞的黏附、DNA 合成量、糖胺聚糖分泌量和MTT 活性的影响。实验结果表明，当培养液中透明质酸的浓度小于0.1%时，对成纤维细胞的黏附和生长增殖有明显的促进作用，当培养液中透明质酸的浓度大于0.1%时，对成纤维细胞的黏附和生长增殖有很强的抑制作用。

Abstract

Hyaluronic acid

a component of the glycosaminoglycans in extracellular matrix

has a special physicochemical properties and influences several cellular functions such as adhesion

migration

and proliferation . The object of this study was to investigate the relationship between the concentrations of hyaluronic acid solutions and the adhesion and proliferation behaviors of human fibroblasts .Hyaluronic acid solutions with different concentrations were added to cell culture medium separately .Results demonstrated that it was only the concentration of hyaluronic acid solutions in a certain range (0.01 %~ 0.1 %)

that could promote the cell adhesion

DNA synthesizing

GAG secreting and cell proliferation

while the concentrations of hyaluronic acid solutions were above 0.1 %it would either reduce or even inhibit these behaviors .

关键词

透明质酸浓度成纤维细胞黏附增殖

Keywords

Hyaluronic acidConcentrationFibroblastsAdhesionProliferation

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