Peptide nucleic acid (PNA) with peptide linkages as its backbone is a brand new kind of compounds which are developed in recent years.It is an analog of DNA in molecular structure.PNA can hybridize DNA and RNA specifically

thus it can be used as probes.Compared with traditional DNA probes

PNA probes enjoy higher hybrid-stabilization

better specificity

and it can hybridize target substance under low salt ion concentration.This paper makes a systemic comparison between PNA probe and DNA probe in the aspects of probes preparation

hybridization specificity

hybridization kinetics and biosensors applications.