Progress in Effect of Additives in DNA Separation by Capillary Electrophoresis

Dan ZHOU; Yan-mei WANG

doi:10.14028/j.cnki.1003-3726.2006.10.012

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Progress in Effect of Additives in DNA Separation by Capillary Electrophoresis

更新时间：2022-09-18

- Progress in Effect of Additives in DNA Separation by Capillary Electrophoresis
- Polymer Bulletin Vol. 19, Issue 10, Pages: 76-81(2006)
- 作者机构：
  
  中国科学技术大学高分子科学与工程系，合肥　230026
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2006.10.012
  CLC：
- Published：2006-10，
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Dan ZHOU, Yan-mei WANG. Progress in Effect of Additives in DNA Separation by Capillary Electrophoresis. [J]. Polymer Bulletin 19(10):76-81(2006)
DOI：

Dan ZHOU, Yan-mei WANG. Progress in Effect of Additives in DNA Separation by Capillary Electrophoresis. [J]. Polymer Bulletin 19(10):76-81(2006) DOI： 10.14028/j.cnki.1003-3726.2006.10.012.

摘要

使用无胶筛分介质的毛细管电泳（包括毛细管阵列电泳和微芯片电泳）是最重要的DNA 分离技术之一。在低粘度的无胶筛分介质中加入某种添加剂是一种有效且简单的克服填充困难和提高DNA 分离性能的方法。本文就各种添加剂（如多羟基化合物、粘土、金纳米粒子、乳胶粒等）对提高无胶筛分介质中DNA 的分离性能的作用进行了综述。

Abstract

The capillary electrophoresis (including capillary array electrophoresis--CAE and microchip electrophoresis--MCE) using non-gel sieving matrices is one of the most important techniques for DNA separation. Addition of certain additives into low viscous non-gel sieving matrices is an efficient and simple method to overcome the difficulty of filling capillaries and enhance DNA separation.This paper reviews the effect of additives (such as polyhydroxy compounds

montmorillonite clay

gold nanoparticles (GNPs)

PEGylated-latex) on improved DNA separation by capillary electrophoresis in non-gel sieving matrices.

关键词

毛细管电泳DNA 分离无胶筛分介质添加剂作用

Keywords

Capillary electrophoresisDNA separationNon-gel sieving matricesAdditivesEffect

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Institute of Safety Science and Engineering, South China University of Technology

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