Study on the Enzymatic Hydrolysis of Corn Straw with Compound Enzymes after Acid Pretreated

Ling-yan CAI; Yu-long MA; Xiao-xia MA

doi:10.14028/j.cnki.1003-3726.2016.01.009

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Study on the Enzymatic Hydrolysis of Corn Straw with Compound Enzymes after Acid Pretreated

更新时间：2022-09-12

- Study on the Enzymatic Hydrolysis of Corn Straw with Compound Enzymes after Acid Pretreated
- Polymer Bulletin Vol. 29, Issue 1, Pages: 60-67(2016)
- 作者机构：
  
  1.宁夏大学化学化工学院，银川 750021
  2.宁夏大学能源化工国家重点实验室培育基地，银川 750021
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2016.01.009
  CLC：
- Published：20 January 2016，
  
  Received：23 April 2015，
  
  Revised：31 August 2015，
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Ling-yan CAI, Yu-long MA, Xiao-xia MA. Study on the Enzymatic Hydrolysis of Corn Straw with Compound Enzymes after Acid Pretreated. [J]. Polymer Bulletin 29(1):60-67(2016)
DOI：

Ling-yan CAI, Yu-long MA, Xiao-xia MA. Study on the Enzymatic Hydrolysis of Corn Straw with Compound Enzymes after Acid Pretreated. [J]. Polymer Bulletin 29(1):60-67(2016) DOI： 10.14028/j.cnki.1003-3726.2016.01.009.

摘要

以玉米秸秆为研究对象

经过2%硫酸预处理后

利用果胶酶、

-葡萄糖苷酶、纤维素酶三种酶协同酶解

以提高玉米秸秆的酶解产糖量。结果表明:当酶解时间为48h

果胶酶、

-葡萄糖苷酶、纤维素酶分别为45U/mL、30U/mL、60U/mL时

葡萄糖、木糖和酶水解得率分别为67.83%、3.25%、73.65%

相比纤维素酶单一酶解的葡萄糖、木糖和酶水解得率分别提高了65.04%、20.82%、65.06%。分步糖化发酵5天后

相比单一酶解发酵乙醇含量提高了72.5%。说明利用三种酶复合处理

能明显提高酶解产糖量。研究结果为玉米秸秆转化为可发酵糖技术的研究提供重要参考。

Abstract

The aim of this study was to improve the yields of sugar with enzymatic hydrolysis on 2% sulfuric acid pretreated corn straw using pectinase

-glucosidase and cellulase. The results showed that the yields of glucose

xylose

and enzyme hydrolysis were 67.83%

3.25%

and 73.65%

respectively

when the pretreated corn straw was hydrolyzed using pectinase

-glucosidase

and cellulase were 45U/mL

30U/mL and 60U/mL for a period of 48 h. Compared with single cellulose enzymatic hydrolysis

the yields of glucose

xylose and enzymatic hydrolysis with compound enzymes increased to 65.04%

20.82%

and 65.06%. The yields of ethanol with compound enzymes increased to 72.5% in the step by step fermentation for 5 days. These results suggest that the mixture of three kind of enzyme can obviously increase the yield of sugar with enzymatic hydrolysis. The research results provide an important reference for the research of corn straw into fermentable sugar technology.

关键词

生物质玉米秸秆酸水解酶解

Keywords

BiomassCorn strawAcid hydrolysisEnzymatic hydrolysis

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