The New Method of Calculating the Molecular Weight and Distribution of Macromolecules in GPC Test by Using Relative Time Method

Ya-meng YU; Chun-xiu GU; Yin-cong BAO

doi:10.14028/j.cnki.1003-3726.2016.011.006

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The New Method of Calculating the Molecular Weight and Distribution of Macromolecules in GPC Test by Using Relative Time Method

更新时间：2022-09-12

- The New Method of Calculating the Molecular Weight and Distribution of Macromolecules in GPC Test by Using Relative Time Method
- Polymer Bulletin Vol. 29, Issue 11, Pages: 54-59(2016)
- 作者机构：
  
  北京联合大学生物化学工程学院, 生物质废弃物资源化利用北京市重点实验室, 北京 100023
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2016.011.006
  CLC：
- Published：20 November 2016，
  
  Received：30 May 2016，
  
  Revised：18 September 2016，
扫描看全文
Ya-meng YU, Chun-xiu GU, Yin-cong BAO. The New Method of Calculating the Molecular Weight and Distribution of Macromolecules in GPC Test by Using Relative Time Method. [J]. Polymer Bulletin 29(11):54-59(2016)
DOI：

Ya-meng YU, Chun-xiu GU, Yin-cong BAO. The New Method of Calculating the Molecular Weight and Distribution of Macromolecules in GPC Test by Using Relative Time Method. [J]. Polymer Bulletin 29(11):54-59(2016) DOI： 10.14028/j.cnki.1003-3726.2016.011.006.

摘要

在凝胶渗透色谱（GPC）对大分子的分子量和分子量分布的测试中，本研究利用小分子乙二醇在系统中出峰时间恒定的现象，采用乙二醇的出峰时间作为标识物，将GPC的色谱图转化为相对时间，再以相对时间建立校正曲线，作为计算分子量和分子量分布的依据。此方法解决了在测试色谱图的记录、校准曲线的建立和分子量的计算中以实际时间来表示和计算分子量时，由于GPC泵的重复性不稳定、色谱柱柱效的下降、仪器更换过不同的色谱柱等因素造成的使校正曲线出现偏差、分子量计算结果出现误差等问题。

Abstract

In the test of the macromolecular weight and molecular weight distribution by Gel permeation chromatography(GPC)

according to the phenomenon that the micromolecule ethylene glycol’s peak time is constant in the system

this research used ethylene glycol’s peak time as a marker

The chromatographs under the relative time are used to establish a calibration curve which istransformed from those under the absolute time. This project solves the problems caused by the repeatability of GPC instrument’s instability

the declination of chromatographic column efficiency and the replacement of chromatographic column in the records of chromatogram

the establishment of calibration curve and the calculation of molecular weight under the real time.

关键词

凝胶渗透色谱相对时间标准曲线

Keywords

Gel permeation chromatographyRelative timeCalibration curve

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