Progress in Application of Dynamic and Static Light Scattering on Protein

Ke LIU; Jin-yan GAO; Jin YUAN; Hong-bing CHEN; Ping TONG

doi:10.14028/j.cnki.1003-3726.2016.012.003

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Progress in Application of Dynamic and Static Light Scattering on Protein

更新时间：2022-09-12

- Progress in Application of Dynamic and Static Light Scattering on Protein
- Polymer Bulletin Vol. 29, Issue 12, Pages: 16-21(2016)
- 作者机构：
  
  1.南昌大学食品科学与技术国家重点实验室, 南昌 330047
  2.南昌大学食品学院, 南昌 330047
  3.南昌大学中德联合研究院, 南昌 330047
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2016.012.003
  CLC：
- Published：20 December 2016，
  
  Received：13 June 2016，
  
  Revised：04 October 2016，
扫描看全文
Ke LIU, Jin-yan GAO, Jin YUAN, Hong-bing CHEN, Ping TONG. Progress in Application of Dynamic and Static Light Scattering on Protein. [J]. Polymer Bulletin 29(12):16-21(2016)
DOI：

Ke LIU, Jin-yan GAO, Jin YUAN, Hong-bing CHEN, Ping TONG. Progress in Application of Dynamic and Static Light Scattering on Protein. [J]. Polymer Bulletin 29(12):16-21(2016) DOI： 10.14028/j.cnki.1003-3726.2016.012.003.

摘要

动静态光散射技术是研究蛋白质等大分子物质尺寸、分布、形态及构象的一种重要手段，以其快速、便捷、对样品无干扰等特点，逐步深入到生化、物理、医药病理等领域，具有良好的应用前景。本文简要阐述了光散射技术的发展，主要介绍了动静态光散射技术的原理，从流体力学半径、重均分子量、均方根回旋半径以及渗透第二维里系数4个重要的物理参数出发，对近年来动静态光散射技术在蛋白质研究中的应用进行了调研和综述，并对该技术的发展进行了展望。

Abstract

Dynamic and static light scattering (DLS and SLS) are important methods for studying the sizes

shapes and conformation of macromolecules. They have been deeply applied in biochemistry

physics

medical pathology and other fields. In this article

the principles for DLS and SLS were introduced

and in addition

recent researches for application of DLS and SLS on proteins were investigated and reviewed by analysing the hydrodynamic radius

weight-average molecular weight

radius of gyration and second virial coefficient

and future development of application of DLS and SLS was prospected.

关键词

动静态光散射蛋白质基本原理应用

Keywords

Dynamic and static light scatteringProteinPrincipleApplication

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