Qiang TANG, Hong ZHANG, Bao-han ZHOU, Bao-ming XU. Construction of Photo-responsive Gene Delivery System Based on β-Cyclodextrin and Azobenzene Derivatives. [J]. Polymer Bulletin 33(3):40-46(2020)
DOI:
Qiang TANG, Hong ZHANG, Bao-han ZHOU, Bao-ming XU. Construction of Photo-responsive Gene Delivery System Based on β-Cyclodextrin and Azobenzene Derivatives. [J]. Polymer Bulletin 33(3):40-46(2020) DOI: 10.14028/j.cnki.1003-3726.2020.03.006.
Construction of Photo-responsive Gene Delivery System Based on β-Cyclodextrin and Azobenzene Derivatives
HPEI-CDs/Azo-PEG/DNA polyplexes was prepared by β-cyclodextrin modified with polyethyleneimine (HPEI-CDs) and azobenzene-conjugated with polyethylene glycol (Azo-PEG). The stability of its physiological salt condition
size
morphology and photo response were studied. The results showed that compared with HPEI-CDs/DNA polyplexes
the introduction of PEG significantly improved the stability under physiological salt condition. With the synergistic effect of PEG shell charge screening and CD hydrogen bonding
the surface potential of polyplexes was only +3mV
uniform and spherical microparticles were achieved. The surface potential of HPEI-CDs/Azo-PEG/DNA increased rapidly under 365nm wavelength illumination. With the extension of illumination time
ζ potential eventually increased to a level close to that of HPEI-CDs/DNA
it indicated that azobenzene (Azo) was transformed from trans to cis-structure
light irradiation made Azo-CD inclusion complexes disassemble and lead to PEG shell detachment.
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