The primary consideration in wound treatment is hemostasis and regulating inflammation

so the development of hemostatic and anti-inflammatory dressings is of great significance. In this study

two kinds of hydrogels based on collagen were prepared and their hemostatic and anti-inflammatory effects were tested. Collagen was compounded with chondroitin sulfate A (CSA) and chondroitin sulfate C (CSC)

which have anti-inflammatory effects

respectively

and the resulting complex was chemically cross-linked with aldehyde benzoic acid modified polyethylene glycol 6000 (APG6000) to form hydrogels. The hydrogel had a sudden release of chondroitin sulfate A or chondroitin sulfate C

which had the potential of rapid anti-inflammatory when applied to skin wounds. The pores of the hydrogels obversed by the scanning electron microscope (SEM) were compact and uniform. The results of swelling experiment showed favorable water absorption. Both of above properties were beneficial to absorb tissue exudation when applying hydrogels to wound. The results of MTT and living cell staining experiments reflected that the two hydrogels contributed to proliferation of macrophages and fibroblasts. The hemolysis rate of the hydrogels was lower than 1.5%

the blood clotting index (BCI) decreased significantly

and the hemagglutination effect was positive. These results indicated that the two hydrogels have the potential as hemostatic and anti-inflammatory dressings.