The polymerization condition plays a key role in preparing functional microspheres. In this paper

functional microspheres with impedance protein non-specific adsorption were prepared by the dispersion copolymerization of styrene

functional monomer and allyloxy polyethyleneglycol (APEG) under different polymerization conditions. The particle size

size distribution and functional group of these microspheres were measured by Fourier transform infrared (FT-IR)

dynamic light scattering (DLS) and scanning electron microscopy (SEM). It was evaluated resistance non-specific adsorption by mean of immobilized protein on two kinds of functional microspheres. The experimental results indicated that APEG could act as a functional monomer to obtain functional microspheres with narrow size distribution under a certain condition. Moreover

it was found that poly (APEG) could act as a stabilizer in dispersion copolymerization when its molecule weight increased a certain value by self-polymerization under dispersion medium with water. Furthermore

the adsorption capacity of bovine serum albumin (BSA) per gram of P(St-

co

-APEG) microspheres was 0.66 mg

while that of BSA per gram of P(St-

co

-GMA) microspheres was 4.8 mg. In a word

APEG functional microspheres with resistance to protein adsorption were successfully prepared by dispersion copolymerization

via

optimizing the polymerization condition.