Thermo-sensitive Pickering Emulsions Based on Chitosan/Sodium Hyaluronate Colloids and Their Application in Biocatalysis

HU Yun; WENG Min-min; XIA Chun-miao; GUO Xin-miao; HUO Hui-min; CHENG Xiu-cai; ZHANG Rong-li

doi:10.14028/j.cnki.1003-3726.2023.05.006

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Thermo-sensitive Pickering Emulsions Based on Chitosan/Sodium Hyaluronate Colloids and Their Application in Biocatalysis

更新时间：2023-03-06

- Thermo-sensitive Pickering Emulsions Based on Chitosan/Sodium Hyaluronate Colloids and Their Application in Biocatalysis
- Polymer Bulletin Vol. 36, Issue 5, Pages: 604-616(2023)
- 作者机构：
  
  安徽工程大学化学与环境工程学院，芜湖　241000
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2023.05.006
  CLC：
- Published：20 May 2023，
  
  Received：12 July 2022，
  
  Revised：10 September 2022，
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HU Yun, WENG Min-min, XIA Chun-miao, GUO Xin-miao, HUO Hui-min, CHENG Xiu-cai, ZHANG Rong-li. Thermo-sensitive Pickering Emulsions Based on Chitosan/Sodium Hyaluronate Colloids and Their Application in Biocatalysis. [J]. Polymer Bulletin 36(5):604-616(2023)
DOI：

HU Yun, WENG Min-min, XIA Chun-miao, GUO Xin-miao, HUO Hui-min, CHENG Xiu-cai, ZHANG Rong-li. Thermo-sensitive Pickering Emulsions Based on Chitosan/Sodium Hyaluronate Colloids and Their Application in Biocatalysis. [J]. Polymer Bulletin 36(5):604-616(2023) DOI： 10.14028/j.cnki.1003-3726.2023.05.006.

摘要

本文以负载脂肪酶的自组装胶体粒子为乳化剂

以十六烷为油相制备Pickering乳液

通过温度调控乳液的稳定性

提高脂肪酶的循环使用性能。首先

在荷正电的壳聚糖(CS)溶液中依次加入脂肪酶(CRL)和荷负电的天然大分子透明质酸钠(HA)

利用壳聚糖与透明质酸钠之间的静电作用自组装形成脂肪酶@壳聚糖/透明质酸钠胶体(CRL@CS/HA CPs)

将脂肪酶负载于胶体粒子内

提高CRL@CS/HA CPs的稳定性;以相变材料十六烷为油相

在高速均质作用下将CRL@CS/HA CPs组装到十六烷-水界面

构建温敏性Pickering乳液

应用于脂肪酶的界面催化;通过改变温度

诱导油相发生凝固-溶解实现破乳

回收CRL@CS/HA CPs

提高脂肪酶的循环使用性能。

Abstract

In this paper

a thermo-sensitive Pickering emulsion was prepared using self-assembled colloidal particles loaded-lipase as emulsifiers and n-hexadecane as oil phase. Through changing the temperature

the lipase at the interface of Pickering emulsion droplets is easy to be recycled. Firstly

lipase and negatively charged sodium hyaluronic acid (HA) were added into positively charged chitosan (CS) solution. The self-assembled lipase@chitosan/sodium hyaluronate colloidal particles (CRL@CS/HA CPs) are formed because of the electrostatic interactions between CS

HA and CRL. The lipase was loaded in the colloidal particles and its stability was improved. To construct a thermo-sensitive Pickering emulsion

the prepared CRL@CS/HA CPs was used as stabilizer

and n-hexadecane as oil phase. By changing the temperature

the oil phase undergoes solidification and dissolution

realizing the separation of oil and water and thus the recycling of CRL@CS/HA CPs.

关键词

自组装胶体脂肪酶Pickering乳液温敏性乳液生物催化

Keywords

Self-assembled colloidLipasePickering emulsionThermo-sensitive emulsionBiocatalysis

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安徽工程大学化学与环境工程学院

Faculty of Materials Science and Chemical Engineering, Ningbo University

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Graduate University of Chinese Academy of Sciences

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