Preparation and Evaluation of UV Cross-linked Marine Collagen/Hyaluronic Acid Composite Hydrogel

WU Yue; LI Yan-yan; FU Ying-kun; PAN Hong-fu; CHANG Cong; ZHENG Zi-qi; ZHANG Zheng-nan; LI Ri-wang; DUAN Shu-xia; AO Ning-jian

doi:10.14028/j.cnki.1003-3726.2023.05.009

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Preparation and Evaluation of UV Cross-linked Marine Collagen/Hyaluronic Acid Composite Hydrogel

更新时间：2023-03-06

- Preparation and Evaluation of UV Cross-linked Marine Collagen/Hyaluronic Acid Composite Hydrogel
- Polymer Bulletin Vol. 36, Issue 5, Pages: 630-642(2023)
- 作者机构：
  
  1.暨南大学生命科学技术学院，广州　510632
  2.广东省教育厅生物材料重点实验室，广州　510632
  3.河南亚都实业有限公司，新乡　453000
  4.河南省医用防护用品重点实验室，新乡　453000
- 作者简介：
- 基金信息：
- DOI：10.14028/j.cnki.1003-3726.2023.05.009
  CLC：
- Published：20 May 2023，
  
  Received：10 August 2022，
  
  Revised：15 September 2022，
扫描看全文
WU Yue, LI Yan-yan, FU Ying-kun, PAN Hong-fu, CHANG Cong, ZHENG Zi-qi, ZHANG Zheng-nan, LI Ri-wang, DUAN Shu-xia, AO Ning-jian. Preparation and Evaluation of UV Cross-linked Marine Collagen/Hyaluronic Acid Composite Hydrogel. [J]. Polymer Bulletin 36(5):630-642(2023)
DOI：

WU Yue, LI Yan-yan, FU Ying-kun, PAN Hong-fu, CHANG Cong, ZHENG Zi-qi, ZHANG Zheng-nan, LI Ri-wang, DUAN Shu-xia, AO Ning-jian. Preparation and Evaluation of UV Cross-linked Marine Collagen/Hyaluronic Acid Composite Hydrogel. [J]. Polymer Bulletin 36(5):630-642(2023) DOI： 10.14028/j.cnki.1003-3726.2023.05.009.

摘要

海星具有很强的再生能力

体壁中含有丰富的胶原蛋白(SSC)。以甲基丙烯酸化透明质酸(HAMA)和甲基丙烯酸化胶原蛋白(SSCMA)为原料

复合海洋源小球藻生长因子(CGF)

采用紫外光交联法制备复合水凝胶

并对复合水凝胶的力学性能、溶胀性、降解性能、微观结构进行分析

同时验证了复合水凝胶对细胞的毒性及增殖作用。通过扫描电镜观察到SSCMA/HAMA/CGF复合水凝胶具有明显的孔状结构

随着HAMA比例的增加

水凝胶的力学性能也得到了提高。当HAMA 的添加量为60%时

复合水凝胶在24 h内的溶胀率最大

可达到其自身重量的5 倍左右。经过30 天

SSCMA/HAMA/CGF-75 发生最大量的降解。SSCMA/HAMA/CGF复合水凝胶对小鼠表皮成纤维细胞(L929)无毒副作用

表现出良好的细胞相容性

且对小鼠胚胎成纤维细胞(NIH-3T3)具有明显的增殖作用

其中SSCMA/HAMA/CGF-60 对细胞的增殖率最大

可达129.70%。

Abstract

Starfish have strong regenerative abilities and are rich in collagen (SSC) in their body walls. Using methacrylated hyaluronic acid (HAMA) and methacrylated collagen (SSCMA) as raw materials

combined with marine source Chlorella growth factor (CGF)

the composite hydrogel was prepared by ultraviolet cross-linking method

and the mechanical properties

swelling properties

degradation properties and microstructure of the composite hydrogels were analyzed

and the toxicity and proliferation effects of the composite hydrogels on cells were also verified. The SSCMA/HAMA/CGF composite hydrogels were observed by scanning electron micros-copy to have an obvious pore-like structure

and the mechanical properties of the hydrogels were also improved with the increase of the HAMA ratio. When the addition amount of HAMA is 60%

the swelling rate of the composite hydrogel within 24 h is the largest

which can reach about 5 times of its own weight. After 30 days of degradation

SSCMA/HAMA/CGF-75 undergoes the greatest amount of degradation. SSCMA/HAMA/CGF composite hydrogel has no toxic and side effects on mouse epidermal fibroblasts (L929)

shows good cytocompatibility

and has obvious proliferation effect on mouse embryonic fibroblasts (NIH-3T3)

among which SSCMA/HAMA/CGF-60 had the highest cell proliferation rate

reaching 129.70%.

关键词

海星壳胶原蛋白透明质酸光交联水凝胶细胞毒性

Keywords

Starfish shell collagenHyaluronic acidPhotocrosslinkingHydrogelCytotoxicity

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