3D打印水凝胶支架构建血管化组织/器官研究进展
Research Progress on Constructing Vascularized Tissues/Organs
via 3D-Printed Hydrogel Scaffoldss- 高分子通报 2025年38卷第12期 页码：1776-1788
- 作者机构：
华南理工大学国家人体组织功能重建工程技术研究中心，广州 510006
- 作者简介：
E-mail: chaimuyuan@scut.edu.cn
E-mail: shxt@scut.edu.cn
- 基金信息：国家自然科学基金(52525211)
DOI：10.14028/j.cnki.1003-3726.2025.25.236
中图分类号：收稿：2025-08-20，
录用：2025-09-30，
网络出版：2025-11-12，
纸质出版：2025-12-20
- 稿件说明：
移动端阅览
卜昊霖, 柴牧原, 施雪涛. 3D打印水凝胶支架构建血管化组织/器官研究进展. 高分子通报, 2025, 38(12), 1776–1788.
Bu, H. L.; Chai, M. Y.; Shi, X. T. Research progress on constructing vascularized tissues/organs via 3D-printed hydrogel scaffoldss. Polym. Bull. (in Chinese), 2025, 38(12), 1776–1788.
卜昊霖, 柴牧原, 施雪涛. 3D打印水凝胶支架构建血管化组织/器官研究进展. 高分子通报, 2025, 38(12), 1776–1788. DOI： 10.14028/j.cnki.1003-3726.2025.25.236.
Bu, H. L.; Chai, M. Y.; Shi, X. T. Research progress on constructing vascularized tissues/organs via 3D-printed hydrogel scaffoldss. Polym. Bull. (in Chinese), 2025, 38(12), 1776–1788. DOI： 10.14028/j.cnki.1003-3726.2025.25.236.
摘要
3D打印水凝胶支架凭借其结构可控性强、生物相容性优异的特点，已成为构建血管化组织/器官的重要方法。目前，借助数字光处理、挤出式打印等技术，已实现分级血管网络的精准构建，并在心脏、肝脏等器官特异性模型方面取得了突破性进展，如可缝合血管支架、仿生肝血窦芯片等。然而，当前相关类器官构建仍面临一些瓶颈问题，如大尺寸组织(厚度>500 μm)缺氧、生物墨水标准化不足等。未来，需要依托人工智能(AI)优化打印路径，融合微流控技术构建可灌流模型，以推动血管化组织的临床转化。
Abstract
3D-printed hydrogel scaffolds have become important tools for constructing vascularized tissues/organs owing to their high structural controllability and excellent biocompatibility. Currently
technologies such as digital light processing and extrusion-based printing have enabled the precise construction of hierarchical vascular networks
achieving breakthroughs in organ-specific models
such as the heart and liver
including suturable vascular scaffolds and bionic hepatic sinusoid chips. However
the current construction of relevant organoids still faces bottlenecks
such as hypoxia in large tissues (thickness > 500 μm) and insufficient standardization of bioinks. In this future
it will be necessary to rely on AI to optimize printing paths and integrate microfluidic technology to construct perfusable models
thereby promoting the clinical translation of vascularized tissues.
关键词
Keywords
references
van Kesteren, S. ; Shen, X. T. ; Aldeghi, M. ; Isa, L . Printing on particles: combining two-photon nanolithography and capillary assembly to fabricate multimaterial microstructures . Adv. Mater. , 2023 , 35 ( 11 ), 2207101 .
Grigoryan, B. ; Paulsen, S. J. ; Corbett, D. C. ; Sazer, D. W. ; Fortin, C. L. ; Zaita, A. J. ; Greenfield, P. T. ; Calafat, N. J. ; Gounley, J. P. ; Ta, A. H. ; Johansson, F. ; Randles, A. ; Rosenkrantz, J. E. ; Louis-Rosenberg, J. D. ; Galie, P. A. ; Stevens, K. R. ; Miller, J. S . Multivascular networks and functional intravascular topologies within biocompatible hydrogels . Science , 2019 , 364 ( 6439 ), 458 – 464 .
Attalla, R. ; Puersten, E. ; Jain, N. ; Selvaganapathy, P. R . 3D bioprinting of heterogeneous bi- and tri-layered hollow channels within gel scaffolds using scalable multi-axial microfluidic extrusion nozzle . Biofabrication , 2019 , 11 ( 1 ), 015012 .
Liang, Q. F. ; Gao, F. ; Zeng, Z. W. ; Yang, J. R. ; Wu, M. M. ; Gao, C. J. ; Cheng, D. L. ; Pan, H. B. ; Liu, W. G. ; Ruan, C. S . Coaxial scale-up printing of diameter-tunable biohybrid hydrogel microtubes with high strength, perfusability, and endothelialization . Adv. Funct. Mater. , 2020 , 30 ( 43 ), 2001485 .
Joshi, A. ; Choudhury, S. ; Gugulothu, S. B. ; Visweswariah, S. S. ; Chatterjee, K . Strategies to promote vascularization in 3D printed tissue scaffolds: trends and challenges . Biomacromolecules , 2022 , 23 ( 7 ), 2730 – 2751 .
Hinton, T. J. ; Jallerat, Q. ; Palchesko, R. N. ; Park, J. H. ; Grodzicki, M. S. ; Shue, H. J. ; Ramadan, M. H. ; Hudson, A. R. ; Feinberg, A. W . Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels . Sci. Adv. , 2015 , 1 ( 9 ), e1500758 .
Lee, A. ; Hudson, A. R. ; Shiwarski, D. J. ; Tashman, J. W. ; Hinton, T. J. ; Yerneni, S. ; Bliley, J. M. ; Campbell, P. G. ; Feinberg, A. W . 3D bioprinting of collagen to rebuild components of the human heart . Science , 2019 , 365 ( 6452 ), 482 – 487 .
Kolesky, D. B. ; Truby, R. L. ; Gladman, A. S. ; Busbee, T. A. ; Homan, K. A. ; Lewis, J. A . 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs . Adv. Mater. , 2014 , 26 ( 19 ), 3124 – 3130 .
Skylar-Scott, M. A. ; Uzel, S. G. M. ; Nam, L. L. ; Ahrens, J. H. ; Truby, R. L. ; Damaraju, S. ; Lewis, J. A . Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels . Sci. Adv. , 2019 , 5 ( 9 ), eaaw2459 .
Ye, T. ; Chai, M. Y. ; Wang, Z. X. ; Shao, T. R. ; Liu, J. ; Shi, X. T . 3D-printed hydrogels with engineered nanocrystalline domains as functional vascular con-structs . ACS Nano , 2024 , 18 ( 37 ), 25765 – 25777 .
Wang, Z. X. ; Liu, X. M. ; Shi, X. T. ; Wang, Y. J . Microvascular network based on the Hilbert curve for nutrient transport in thick tissue . Regen. Biomater. , 2024 , 11 , rbae094 .
Mirdamadi, E. ; Tashman, J. W. ; Shiwarski, D. J. ; Palchesko, R. N. ; Feinberg, A. W . Fresh 3D bioprinting a full-size model of the human heart . ACS Biomater. Sci. Eng. , 2020 , 6 ( 11 ), 6453 – 6459 .
Lee, S. ; Sani, E. S. ; Spencer, A. R. ; Guan, Y. ; Weiss, A. S. ; Annabi, N . Human-recombinant-elastin-based bioinks for 3D bioprinting of vascularized soft tissues . Adv. Mater. , 2020 , 32 ( 45 ), 2003915 .
Fang, Y. C. ; Guo, Y. H. ; Wu, B. Y. ; Liu, Z. B. ; Ye, M. ; Xu, Y. Y. ; Ji, M. K. ; Chen, L. ; Lu, B. C. ; Nie, K. J. ; Wang, Z. X. ; Luo, J. B. ; Zhang, T. ; Sun, W. ; Xiong, Z . Expanding embedded 3D bioprinting capability for engineering complex organs with freeform vascular networks . Adv. Mater. , 2023 , 35 ( 22 ), 2205082 .
Lu, B. C. ; Ye, M. ; Xia, J. J. ; Zhang, Z. R. ; Xiong, Z. ; Zhang, T . Electrical stimulation promotes the vascularization and functionalization of an engineered biomimetic human cardiac tissue . Adv. Healthc. Mater. , 2023 , 12 ( 19 ), 2300607 .
Coulter, F. B. ; Schaffner, M. ; Faber, J. A. ; Rafsanjani, A. ; Smith, R. ; Appa, H. ; Zilla, P. ; Bezuidenhout, D. ; Studart, A. R. . Bioinspired heart valve prosthesis made by silicone additive manufacturing . Matter , 2019 , 1 ( 1 ), 266 – 279 .
Sokmen, S. ; Cakmak, S. ; Oksuz, I . 3D printing of an artificial intelligence-generated patient-specific coronary artery segmentation in a support bath . Biomed. Mater. , 2024 , 19 , 035038 .
Carpentier, N. ; Van der Meeren, L. ; Skirtach, A. G. ; Devisscher, L. ; Van Vlierberghe, H. ; Dubruel, P. ; van Vlierberghe, S . Gelatin-based hybrid hydrogel scaffolds: toward physicochemical liver mimicry . Biomacromolecules , 2023 , 24 ( 10 ), 4333 – 4347 .
Maji, S. ; Lee, M. ; Lee, J. ; Lee, J. ; Lee, H . Development of lumen-based perfusable 3D liver in vitro model using single-step bioprinting with composite bioinks . Mater. Today Bio , 2023 , 21 , 100723 .
Yang, H. Y. ; Sun, L. J. ; Pang, Y. ; Hu, D. D. ; Xu, H. F. ; Mao, S. S. ; Peng, W. B. ; Wang, Y. N. ; Xu, Y. Y. ; Zheng, Y. C. ; Du, S. D. ; Zhao, H. T. ; Chi, T. Y. ; Lu, X. ; Sang, X. T. ; Zhong, S. X. ; Wang, X. ; Zhang, H. B. ; Huang, P. Y. ; Sun, W. ; Mao, Y. L . Three-dimensional bioprinted hepatorganoids prolong survival of mice with liver failure . Gut , 2021 , 70 ( 3 ), 567 – 574 .
Sun, L. J. ; Wang, Y. H. ; Zhang, S. Q. ; Yang, H. Y. ; Mao, Y. L . 3D bioprinted liver tissue and disease models: current advances and future perspectives . Biomater. Adv. , 2023 , 152 , 213499 .
Singh, N. K. ; Han, W. ; Nam, S. A. ; Kim, J. W. ; Kim, J. Y. ; Kim, Y. K. ; Cho, D. W . Three-dimensional cell-printing of advanced renal tubular tissue analogue . Biomaterials , 2020 , 232 , 119734 .
An, G. ; Guo, F. X. ; Liu, X. M. ; Wang, Z. F. ; Zhu, Y. ; Fan, Y. ; Xuan, C. K. ; Li, Y. ; Wu, H. K. ; Shi, X. T. ; Mao, C. B . Functional reconstruction of injured corpus cavernosa using 3D-printed hydrogel scaffolds seeded with HIF-1α-expressing stem cells . Nat. Commun. , 2020 , 11 ( 1 ), 2687 .
Chai, M. Y. ; Zhong, W. W. ; Yan, S. T. ; Ye, T. ; Zheng, R. ; Yang, Z. L. ; Shi, X. T . Diffusion-induced phase separation 3D printed scaffolds for dynamic tissue repair . BMEMat , 2024 , 2 ( 3 ), e12119 .
Wang, Z. X. ; Liu, X. M. ; Ye, T. ; Zhai, Z. C. ; Wu, K. ; Kuang, Y. D. ; Ostrovidov, S. ; Shao, D. ; Wang, Y. J. ; Leong, K. W. ; Shi, X. T . 3D-printed perfused models of the penis for the study of penile physiology and for restoring erectile function in rabbits and pigs . Nat. Biomed. Eng. , 2025 , 9 ( 8 ), 1276 – 1289 .
王芳田 , 赵亮 , 郭广生 , 汪夏燕 . 基于微流控芯片的体外血管网络模型的研究进展 . 化学进展 , 2024 , 36 ( 6 ), 840 – 850 .
Shan, B. H. ; Wu, F. G . Hydrogel-based growth factor delivery platforms: strategies and recent advances . Adv. Mater. , 2024 , 36 ( 5 ), 2210707 .
0
浏览量
299
下载量
0
CSCD
- 网络PDF下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备05002797号-16
京公网安备11010802046898号 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies