茯苓碱溶性多糖止血海绵的制备及其止血性能

陈颖; 李皓琛; 黎东儒; 唐克静; 何江城; 李菀

doi:10.14028/j.cnki.1003-3726.2026.26.047

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茯苓碱溶性多糖止血海绵的制备及其止血性能

研究论文 | 更新时间：2026-05-29

- 茯苓碱溶性多糖止血海绵的制备及其止血性能
- Preparation and Hemostatic Performance of Poria cocos Alkali-soluble Polysaccharide Sponges
- 高分子通报 2026年39卷第6期页码：953-966
- 作者机构：
  
  湖北中医药大学药学院　武汉 430065
  武汉中西医结合骨科医院武汉体育学院附属医院　武汉 430079
  湖北时珍实验室　武汉 430065
- 作者简介：
  
  2024333@whsu.edu.cn
  wanLi3055@hbucm.edu.cn
- 基金信息：
  
  国家自然科学基金(81803735);武汉中西医结合骨科医院科研项目(WTFY202304)
- DOI：10.14028/j.cnki.1003-3726.2026.26.047
  中图分类号：
- 收稿：2026-01-28，
  
  录用：2026-03-02，
  
  网络首发：2026-05-08，
  
  纸质出版：2026-06-20
- 稿件说明：
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陈颖, 李皓琛, 黎东儒, 唐克静, 何江城, 李菀. 茯苓碱溶性多糖止血海绵的制备及其止血性能. 高分子通报, 2026, 39(6), 953–966.

Chen, Y.; Li, H. C.; Li, D. R.; Tang, K. J.; He, J. C.; Li, W. Preparation and hemostatic performance of poria cocos alkali-soluble polysaccharide sponges. Polym. Bull. (in Chinese), 2026, 39(6), 953–966.
陈颖, 李皓琛, 黎东儒, 唐克静, 何江城, 李菀. 茯苓碱溶性多糖止血海绵的制备及其止血性能. 高分子通报, 2026, 39(6), 953–966. DOI： 10.14028/j.cnki.1003-3726.2026.26.047.

Chen, Y.; Li, H. C.; Li, D. R.; Tang, K. J.; He, J. C.; Li, W. Preparation and hemostatic performance of poria cocos alkali-soluble polysaccharide sponges. Polym. Bull. (in Chinese), 2026, 39(6), 953–966. DOI： 10.14028/j.cnki.1003-3726.2026.26.047.

摘要

创伤性出血是临床面临的主要挑战之一，亟需开发兼具高效止血性能与良好生物安全性的新型材料。基于茯苓碱溶性多糖(PCAP)作为

-葡聚糖的药用与成胶潜力，本研究以常被废弃的天然多糖PCAP为原料，采用冷冻干燥技术制备了PCAP质量浓度梯度为10~100 g/L的系列海绵，并根据其形态完整性确定了50~100 g/L为可成型的有效质量浓度。扫描电子显微镜(SEM)图像显示PCAP海绵呈现三维多孔结构；物理性能测试证明其具有良好的吸水能力、保水率和水蒸气透过率。体外凝血与溶血实验证实PCAP海绵具有显著的促凝血活性和优异的血液相容性。小鼠断尾和肝脏切口模型的体内止血评价结果显示，质量浓度为70 g/L的PCAP海绵止血效果最优。活化部分凝血活酶时间(APTT)与凝血酶原时间(PT)实验结果显示，PCAP海绵可延长APTT，缩短PT，提示其可能同时影响内源性及外源性凝血途径。组织病理学检查未发现明显的炎症反应，说明其组织相容性良好。上述结果表明，PCAP海绵在止血性能、理化特性及生物相容性方面均表现出良好的综合性能，展现出作为天然多糖基止血海绵敷料的潜在应用价值，为开发绿色安全的止血敷料提供了新策略。

Abstract

Traumatic hemorrhage remains a major clinical challenge

underscoring the urgent need for the development of novel materials that combine excellent hemostatic efficacy with good biosafety. Based on the medicinal potential of

Poria cocos

alkali-soluble polysaccharide (PCAP) and its gelation potential as a

-glucan

this study utilized the commonly discarded natural polysaccharide PCAP as the raw material to prepare a series of sponges with PCAP concentration

gradients of 10–100 g/L

via

freeze-drying. Based on morphological integrity

50–100 g/L was identified as the effective formable concentration range. Scanning electron microscopy (SEM) images revealed that the PCAP sponges exhibited a three-dimensional porous structure

while physical performance tests demonstrated favorable water absorption capacity

water retention rate

and water vapor transmission rate. In vitro coagulation and hemolysis experiments confirmed the significant pro-coagulant activity and excellent hemocompatibility of the PCAP sponges. In vivo hemostatic performance evaluated using mouse tail amputation and liver incision models indicated optimal hemostatic efficacy at a PCAP concentration of 70 g/L. Activated partial thromboplastin time (APTT) and prothrombin time (PT) assay indicated that PCAP sponges prolonged APTT and shortened PT

suggesting a dual modulatory effect on both the intrinsic and extrinsic coagulation pathways. Histopathological analysis revealed no significant inflammatory response

indicating good tissue compatibility. These results demonstrate that PCAP-based sponges exhibit favorable comprehensive properties in terms of hemostatic performance

physicochemical characteristics

and biocompatibility. They show potential application value as a natural polysaccharide-based hemostatic sponge dressing

providing a new strategy for developing green and safe hemostatic dressings.

关键词

Keywords

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