The Layer-by-Layer self-assembly (LbL)of polycations and polyanions into multilayers has emerged as an efficient

versatile

yet simple

technique to create biologically active surfaces .By controlling the assemble condition

bioactive molecules

such as drugs

enzymes

DNA

or proteins

may be incorporated in the multilayer during the polyelecrolyte deposition process .The LbL technique has been recognized as an important means in designing the surfaces of the biomaterials .This paper summarized the progress on the Layer-by-Layer self-assemble technique in the design of the biointerface for cardiovascular device

tissue engineering scaffold

drug delivery systems

and so on .