基于PVA物理交联构建具有分级结构的一体化支架

赵燕芳; 钟美玲; 唐奥奇; 席荣祥; 彭梦霞; 甘德强; 张跃进

doi:10.14028/j.cnki.1003-3726.2024.23.243

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基于PVA物理交联构建具有分级结构的一体化支架

研究论文 | 更新时间：2024-02-06

- 基于PVA物理交联构建具有分级结构的一体化支架
- An Integrated Poly(vinyl alcohol) (PVA) Scaffold with Hierarchical Structure Prepared by Physical Crosslinking
- 高分子通报 2024年37卷第3期页码：385-394
- 作者机构：
  
  华东交通大学材料工程与科学学院，南昌 330013
- 作者简介：
  
  *钟美玲，E-mail: zhongmei121987@163.com
- 基金信息：
  
  国家自然科学基金(52363020;51903084);江西省自然科学基金(20212BAB204005;20202-ACBL214004);江西省教育厅科学技术研究项目-青年项目《介孔纳米管附载可控释放的生物活性因子骨支架研究》(GJJ2200656);江西省自然科学基金重点项目(20232ACB205001);“赣鄱俊才支持计划——主要学科学术和技术带头人培养项目”领军人才（学术类）(20232BCJ22025)
- DOI：10.14028/j.cnki.1003-3726.2024.23.243
  中图分类号：
- 纸质出版日期：2024-03-20，
  
  收稿日期：2023-07-12，
  
  录用日期：2023-09-19
- 稿件说明：
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赵燕芳, 钟美玲,唐奥奇, 席荣祥, 彭梦霞, 甘德强, 张跃进. 基于PVA物理交联构建具有分级结构的一体化支架. 高分子通报, 2024, 37(3), 385–394

Zhao, Y. F.; Zhong, M. L.; Tang, A. Q.; Xi, R. X.; Peng, M. X.; Gan, D. Q.; Zhang, Y. J. An integrated poly(vinyl alcohol) (PVA) scaffold with hierarchical structure prepared by physical crosslinking. Polym. Bull. (in Chinese), 2024, 37(3), 385–394
赵燕芳, 钟美玲,唐奥奇, 席荣祥, 彭梦霞, 甘德强, 张跃进. 基于PVA物理交联构建具有分级结构的一体化支架. 高分子通报, 2024, 37(3), 385–394 DOI： 10.14028/j.cnki.1003-3726.2024.23.243.

Zhao, Y. F.; Zhong, M. L.; Tang, A. Q.; Xi, R. X.; Peng, M. X.; Gan, D. Q.; Zhang, Y. J. An integrated poly(vinyl alcohol) (PVA) scaffold with hierarchical structure prepared by physical crosslinking. Polym. Bull. (in Chinese), 2024, 37(3), 385–394 DOI： 10.14028/j.cnki.1003-3726.2024.23.243.

摘要

具有分级结构的界面组织(如肌腱-骨界面、骨-软骨界面等)在器官的正常发育和功能中扮演着极其重要的作用。其中，肌腱-骨界面组织是最为典型的界面组织之一。为此，本研究采用肌腱-骨界面组织的构建为模型，以明胶、聚乙烯醇(PVA)、羟基磷灰石(HAp)为原料，采用冻融循环技术结合逐层涂覆技术制备具有分级结构的仿肌腱-骨界面一体化支架。该支架由三个部分组成：(i)上层为未矿化的区域，以促进支架与肌腱的整合；(ii)中间层为具有矿物成分梯度和力学性能梯度的区域，以促进应力在肌腱和骨之间的转移；(iii)下层为具有较高力学性能的矿化区域，促进支架与骨的整合。采用红外光谱、力学性能测试、扫描电镜(SEM)、能谱分析(EDS)等手段对支架的结构和性能进行表征。结果表明，采用冻融循环技术可以通过物理交联获得PVA支架，且矿物量-力学，冷冻-解冻循环次数-力学的力学拉伸测试结果表明该支架从上层到下层的力学性能依次呈梯度增加。采用扫描电镜(SEM)观察了材料的整体微观结构，材料层与层之间完美融合，形成具有分级结构的一体化支架。最后，通过能谱分析(EDS)对支架的矿物质含量进行分析，结果表明该支架从上层到下层的矿物质含量依次从无到有，并呈梯度增加。这种仿生一体化支架的设计将在界面组织工程中具有极大的应用前景。

Abstract

Hierarchical interfacial tissues (such as the tendon-bone interface

bone-cartilage interface

etc

.) play an extremely important role in maintaining the development and function of organs. The tendon-bone interface is one of the most typical interfacial tissues. Therefore

in this study

gelatin

poly(vinyl alcohol) (PVA) and hydroxyapatite (HAp) were mixed together

and the coating technology combined with freezing-thawing method was used to prepare the hierarchical integrated scaffold. The scaffold consisted of three regions: (i) the upper layer is an unmineralized area to facilitate the integration of the scaffold into the tendon; (ii) the intermediate layer is a zone with gradients of mineral composition and mechanical properties to facilitate stress transfer between tendon and bone; (iii) the lower layer is a mineralized area with high mechanical properties which promotes the integration of scaffolds and bones. The structure and properties of the scaffold were characterized by infrared spectroscopy

mechanical properties testing

scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The result showed that the PVA scaffold can be obtained by physical crosslinking using freeze-thaw cycle technology

and the mechanical tensile test results of mineral content-mechanics

freeze-thaw cycles-mechanics showed that the mechanical properties of the scaffold from the top to the bottom gradually increase. SEM was used to observe the overall microstructure of the material. The layers of the material fused perfectly

which formed an integrated scaffold with hierarchical structure. Finally

the mineral content of the scaffold was analyzed by EDS. The results showed that the mineral content of the scaffold increased gradually from the upper layer to the lower layer in a gradient manner. This kind of biomimetic scaffold will have great application prospect in interface tissue engineering.

关键词

聚乙烯醇物理交联分级结构冻融循环技术界面组织

Keywords

Poly(vinyl alcohol)Physical crosslinkingHierarchical structureFreezing-thawing methodInterfacial tissues

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