胆固醇疏水改性壳聚糖自聚集纳米微粒的制备及其动物体内分布研究

原续波; 顾鸣岐; 原艳波; 李弘; 王来好; 盛京

doi:10.14028/j.cnki.1003-3726.2005.05.017

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胆固醇疏水改性壳聚糖自聚集纳米微粒的制备及其动物体内分布研究

研究简报 | 更新时间：2022-09-18

- 胆固醇疏水改性壳聚糖自聚集纳米微粒的制备及其动物体内分布研究
- Paration of Self-aggregate Nanoparticles Composed of Cholesterol Hydrophobic- modefied Chitosan and their Destribution in Cephalosome and Abodominal Cavity
- 高分子通报 2005年18卷第5期页码：122-127
- 作者机构：
  
  1.天津大学材料科学与工程学院，天津　300072
  2.山西长治医学院附属医院，长治　046000
  3.南开大学吸附分离功能高分子材料国家重点实验室，天津　300071
- 作者简介：
  
  原续波，37岁，男，副教授，从事生物医用材料研究，Tel：022-27402702，E-mail：xbyuan@eyou.com
- 基金信息：
  
  国家自然科学基金青年基金资助项目(50301008)
- DOI：10.14028/j.cnki.1003-3726.2005.05.017
  中图分类号：
- 纸质出版日期：2005-10，
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原续波, 顾鸣岐, 原艳波, 李弘, 王来好, 盛京. 胆固醇疏水改性壳聚糖自聚集纳米微粒的制备及其动物体内分布研究[J]. 高分子通报, 2005,18(5):122-127.

Xu-bo YUAN, Ming-qi GU, Yan-bo YUAN, Hong LI, Lai-hao WAN, Jing SHENG. Paration of Self-aggregate Nanoparticles Composed of Cholesterol Hydrophobic- modefied Chitosan and their Destribution in Cephalosome and Abodominal Cavity[J]. Polymer Bulletin, 2005,18(5):122-127.
原续波, 顾鸣岐, 原艳波, 李弘, 王来好, 盛京. 胆固醇疏水改性壳聚糖自聚集纳米微粒的制备及其动物体内分布研究[J]. 高分子通报, 2005,18(5):122-127. DOI： 10.14028/j.cnki.1003-3726.2005.05.017.

Xu-bo YUAN, Ming-qi GU, Yan-bo YUAN, Hong LI, Lai-hao WAN, Jing SHENG. Paration of Self-aggregate Nanoparticles Composed of Cholesterol Hydrophobic- modefied Chitosan and their Destribution in Cephalosome and Abodominal Cavity[J]. Polymer Bulletin, 2005,18(5):122-127. DOI： 10.14028/j.cnki.1003-3726.2005.05.017.

摘要

以EDC 为偶联剂，将胆固醇接枝到壳聚糖主链的氨基上，得到疏水改性的双亲性壳聚糖（CS-Chol），利用FT-IR，

H-NMR 对其结构进行表征。CS-Chol 在水相自组装形成自聚集体，通过荧光探针技术研究其自聚集行为，结果显示制得的自聚集体具有较低的CAC（10

－2

mg/mL）。TEM 和DLS 测试显示制得的自聚集体为纳米级颗粒，粒径随胆固醇取代度的减小（4.7~1.1）而增大（180~380nm）。用

99m

Tc 对纳米颗粒进行放射性标记，并利用SPECT 对其在头部和腹腔分布进行分析。耳静脉注射后纳米粒子在粘膜富集区域有一定滞留性，主要是由于壳聚糖具有粘膜特征吸附的性质。

Abstract

Cholesterol hydrophobic-modified chitosan (CS-Chol) were synthesized by coupling cholesterol to the amino groups of chitosan with EDC as catalyzer.The structure of CS-Chol was characterized by FTIR and

H NMR. CS-Chol formed self-aggregates in water

and the self-aggregate behavior were studied by fluorescence probe technique

the results suggested that the self-aggregates had low critical aggregate concentration about 10

-2

mg/mL

which was critical to the stability in vivo.The measurements of transmission electron microscope(TEM) and dynamic light scattering(DLS) indicated that the self-aggregates were nanoparticles

and the diameters increased from 180 to 380nm with the decrease of substitute degree from 4.7 to 1.1. The nanoparticles were radio-labeled with

99m

and their distribution in cephalosome and abdominal cavity was analysized by SPECT.CS-Chol nanoparticles were eliminated from blood and abdominal cavity quickly.Some nanoparticles

however

retained in the mucous membrane rich area after otic vein injection

due to the feature adhesion of chitosan to the mucous membrane.

关键词

胆固醇疏水改性壳聚糖（CS-Chol）自聚集纳米微粒放射性标记体内分布

Keywords

ChitosanCholesterolSelf-aggregateNanoparticlesRadio-labelBiodistribution

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