HB-DAS/PSS multilayers were fabricated through layer-by-layer self-assembly on polyurethane carrier surface. The multilayers fabrication process was characterized by using UV-vis absorption spectroscopy and optical ellipsometry. The biological quality of carries was carefully investigated. Uniform HB-DAS/PSS multilayers on the polyurethane surface could be obtained. The modified polyurethane surface showed good hydrophilicity and the water contact angle decreased as the numbers of the multilayers increased. The modified carriers showed good stability