PLLA/PVP纤维三维多孔支架的制备及细胞相容性研究

刘瑞来; 齐小宝; 赵瑨云; 付兴平

doi:10.14028/j.cnki.1003-3726.2019.04.006

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PLLA/PVP纤维三维多孔支架的制备及细胞相容性研究

研究简报 | 更新时间：2022-09-02

- PLLA/PVP纤维三维多孔支架的制备及细胞相容性研究
- Three-dimensional Porous Scaffold Based on Poly(L-lactide acid) Polyvinylpyrrolid Fibers and Its Cellular Biocompatibility
- 高分子通报 2019年32卷第4期页码：40-47
- 作者机构：
  
  福建省生态产业绿色技术重点实验室，武夷学院生态与资源工程学院，武夷山 354300
- 作者简介：
  
  *刘瑞来（1984-），男，博士，副教授，主要从事功能高分子材料研究。E-mail: wyulrl@163.com.
- 基金信息：
  
  国家自然科学基金(51406141);福建省自然科学基金(2018J05092);福建省高校产学研合作(2018Y4011);福建省教育厅项目(JAT170595;JT180550;JT180552;JT180553);南平市科技局(N2017DG03);国家大学生创新性实验(201710397014)
- DOI：10.14028/j.cnki.1003-3726.2019.04.006
  中图分类号：
- 纸质出版日期：2019-04-20，
  
  收稿日期：2018-07-18，
  
  修回日期：2018-10-25，
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刘瑞来, 齐小宝, 赵瑨云, 付兴平. PLLA/PVP纤维三维多孔支架的制备及细胞相容性研究[J]. 高分子通报, 2019,32(4):40-47.

Rui-lai LIU, Xiao-bao QI, Jin-yun ZHAO, Xing-ping FU. Three-dimensional Porous Scaffold Based on Poly(L-lactide acid) Polyvinylpyrrolid Fibers and Its Cellular Biocompatibility[J]. Polymer Bulletin, 2019,32(4):40-47.
刘瑞来, 齐小宝, 赵瑨云, 付兴平. PLLA/PVP纤维三维多孔支架的制备及细胞相容性研究[J]. 高分子通报, 2019,32(4):40-47. DOI： 10.14028/j.cnki.1003-3726.2019.04.006.

Rui-lai LIU, Xiao-bao QI, Jin-yun ZHAO, Xing-ping FU. Three-dimensional Porous Scaffold Based on Poly(L-lactide acid) Polyvinylpyrrolid Fibers and Its Cellular Biocompatibility[J]. Polymer Bulletin, 2019,32(4):40-47. DOI： 10.14028/j.cnki.1003-3726.2019.04.006.

摘要

以四氢呋喃（THF）/水为共溶剂，在无其它添加剂条件下，通过低温淬火、萃取、洗涤和干燥得到PLLA/PVP纤维三维多孔支架。采用扫描电镜探讨THF/H

O比例、聚合物浓度、PLLA/PVP比例和淬火温度等条件对纤维支架形貌影响，通过水接触角、吸水率和体外生物活性等测试考察PVP添加对亲水性和生物活性影响。实验结果表明，纤维是由PLLA结晶所形成。PVP加入影响了PLLA结晶，但提高了纤维支架的亲水性。最佳的实验条件为THF/H

O=90 : 10、聚合物浓度9%（wt）、PLLA/PVP=90: 10、淬火温度一10℃，得到了直径为270±73nm均一、规整的纤维三维多孔支架，支架的吸水率、孔隙率和比表面积分别为17.29%、96.36%和13.45m

/g，该孔隙率和比表面积为细胞的粘附、迁移、分化、增殖以及养料的传输提供了很好的条件。

Abstract

Poly (L-lactide acid)/polyvinylpyrrolid three-dimensional nanofibers porous scaffold was prepared by low-temperature quenching

extraction

washing and drying from tetrahydrofuran/water co-solvent without the assistance of other additives. The effects of THF/H

O ratio

concentration

PLLA/PVP ratio and quenching temperature etc. on the morphology of fibrous scaffold were investigated by SEM. The influence of addition of PVP in water absorption and bioactivity were also investigated through water absorption test and in vitro bioactivity test. The results showed that fibrous scaffold was produced by crystallization of PLLA. The addition of PVP impeded the crystallization of PLLA and increased hydrophilia of fibrous scaffold. The optimum test conditions were as followed: THF/H

O=90 : 10

concentration 9%(wt)

PLLA/PVP=90 : 10 and quenching temperature -10℃

the uniform three-dimensional nanofibers porous scaffold with diameter of 270±73 nm were obtained. The water absorption

porosity and specific surface areas of fibrous scaffold were 17.29%

96.36% and 13.45m

respectively. High porosity and specific surface areas will offer good condition of cell adhesion

cell differentiation

cell prolifer and nutrients transport.

关键词

聚乳酸聚乙烯吡咯烷酮多孔支架热致相分离细胞相容性

Keywords

Poly(L-lactide acid)PolyvinylpyrrolidPorous scaffoldThermally induced phase separationCellular biocompatibility

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